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rnascope® 3-plex ls multiplex negative control probe- dapb  (Advanced Cell Diagnostics Inc)


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    Advanced Cell Diagnostics Inc rnascope® 3-plex ls multiplex negative control probe- dapb

    Rnascope® 3 Plex Ls Multiplex Negative Control Probe Dapb, supplied by Advanced Cell Diagnostics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rnascope+3-plex+ls+multiplex+negative+control+probe-+dapb/rnascope+3+plex+negative+control+probe/pmc06838660-42-0-9
    Average 90 stars, based on 1 article reviews
    rnascope® 3-plex ls multiplex negative control probe- dapb - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Glucose-Dependent Insulinotropic Polypeptide Receptor-Expressing Cells in the Hypothalamus Regulate Food Intake"

    Article Title: Glucose-Dependent Insulinotropic Polypeptide Receptor-Expressing Cells in the Hypothalamus Regulate Food Intake

    Journal: Cell Metabolism

    doi: 10.1016/j.cmet.2019.07.013


    Figure Legend Snippet:

    Techniques Used: Virus, Recombinant, Reverse Transcription, RNAscope, Multiplex Assay, Control, Negative Control, Positive Control, Expressing, Isolation, Derivative Assay, Gene Expression, Software

    Related Articles

    Virus:

    Article Title: Glucose-Dependent Insulinotropic Polypeptide Receptor-Expressing Cells in the Hypothalamus Regulate Food Intake
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Goat Polyclonal anti-GLP-1 Santa Cruz Biotechnology Cat # sc-7782; RRID: AB_2107325 Guinea Pig Polycloal anti-insulin Abcam Cat # 7842; RRID: AB_306130 Goat Polyclonal anti-GFP Abcam Cat # 5450; RRID: AB_304897 Rabbit Polyclonal anti-DsRed Takara Bio Cat # 632496; RRID: AB_10013483 Alexa 488 secondary anti-goat Thermo Fisher Scientific Cat # A32814; RRID: AB_2762838 Alexa 555 secondary anti-rabbit Thermo Fisher Scientific Cat # A32794; RRID: AB_2762834 Alexa 633 secondary anti-guinea pig Thermo Fisher Scientific Cat # A-21105; RRID: AB_2535757 Biotinylated donkey anti-goat IgG Millipore Cat # AP180B; RRID: AB_11214009 Bacterial and Virus Strains AAV-hSyn-DIO-hM3D(Gq)-mCherry Addgene Cat # 44361-AAV8 Biological Samples Human hypothalamic brain blocks Cambridge Brain Bank https://www.cuh.nhs.uk/for-public/ cambridge-brain-bank Chemicals, Peptides, and Recombinant Proteins Papain Worthingon/ Lorne Labs Cat # LK003178 SuperScript III Reverse Transcriptase Thermo Fisher Scientific Cat # 18080093 SuperScript II Reverse Transcriptase Thermo Fisher Scientific Cat # 18064014 TaqMan Fast Universal PCR Master Mix Thermo Fisher Scientific Cat # 4364103 Glutamate Sigma Cat # G1251 CCK (octapeptide, sulfated) Tocris Cat # 1166 Hexarelin LKT Laboratories Cat # H1893 Leptin R&D Systems Cat # 498-OB-01M 5-HT Sigma Cat # H9523 Clozapine-N-Oxide Sigma Cat # C0832 Exendin-4 Tocris Cat # 1933 Critical Commercial Assays RNeasy Micro Kit QIAGEN Cat # 74004 RNeasy Plus Micro Kit QIAGEN Cat # 74034 103 Genomics Chromium Single Cell Library Kit v2 103 Genomics Cat # 120234 RNAscope 2.5 LS Multiplex Reagent Kit Advanced Cell Diagnostics Cat # 322800 RNAscope LS 2.5 Probe- Mm-Gipr Advanced Cell Diagnostics Cat # 319128 RNAscope 2.5 LS Probe- Mm-Glp1r Advanced Cell Diagnostics Cat # 418858 RNAscope 3-plex LS Multiplex Control Positive Probe- Mm polr2A, ppib, ubc Advanced Cell Diagnostics Cat # 320888 RNAscope 3-plex LS Multiplex Negative Control Probe- dapB Advanced Cell Diagnostics Cat # 320878 RNAscope 2.5 LS Duplex Reagent Kit Advanced Cell Diagnostics Cat # 322440 RNAscope LS 2.5 Probe- Hs-GLP1R Advanced Cell Diagnostics Cat # 519828 RNAscope 2.5 LS Probe- Hs-GIPR Advanced Cell Diagnostics Cat # 471348 RNAscope 2.5 LS Positive Control Probe- Hs-PPIB Advanced Cell Diagnostics Cat # 313908 RNAscope 2.5 LS Duplex Negative Control Probe- DapB, DapB Advanced Cell Diagnostics Cat # 320758 (Continued on next page) Cell Metabolism 30, 987–996.e1–e6, November 5, 2019 e1

    Bioprocessing:

    Article Title: Glucose-Dependent Insulinotropic Polypeptide Receptor-Expressing Cells in the Hypothalamus Regulate Food Intake
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Goat Polyclonal anti-GLP-1 Santa Cruz Biotechnology Cat # sc-7782; RRID: AB_2107325 Guinea Pig Polycloal anti-insulin Abcam Cat # 7842; RRID: AB_306130 Goat Polyclonal anti-GFP Abcam Cat # 5450; RRID: AB_304897 Rabbit Polyclonal anti-DsRed Takara Bio Cat # 632496; RRID: AB_10013483 Alexa 488 secondary anti-goat Thermo Fisher Scientific Cat # A32814; RRID: AB_2762838 Alexa 555 secondary anti-rabbit Thermo Fisher Scientific Cat # A32794; RRID: AB_2762834 Alexa 633 secondary anti-guinea pig Thermo Fisher Scientific Cat # A-21105; RRID: AB_2535757 Biotinylated donkey anti-goat IgG Millipore Cat # AP180B; RRID: AB_11214009 Bacterial and Virus Strains AAV-hSyn-DIO-hM3D(Gq)-mCherry Addgene Cat # 44361-AAV8 Biological Samples Human hypothalamic brain blocks Cambridge Brain Bank https://www.cuh.nhs.uk/for-public/ cambridge-brain-bank Chemicals, Peptides, and Recombinant Proteins Papain Worthingon/ Lorne Labs Cat # LK003178 SuperScript III Reverse Transcriptase Thermo Fisher Scientific Cat # 18080093 SuperScript II Reverse Transcriptase Thermo Fisher Scientific Cat # 18064014 TaqMan Fast Universal PCR Master Mix Thermo Fisher Scientific Cat # 4364103 Glutamate Sigma Cat # G1251 CCK (octapeptide, sulfated) Tocris Cat # 1166 Hexarelin LKT Laboratories Cat # H1893 Leptin R&D Systems Cat # 498-OB-01M 5-HT Sigma Cat # H9523 Clozapine-N-Oxide Sigma Cat # C0832 Exendin-4 Tocris Cat # 1933 Critical Commercial Assays RNeasy Micro Kit QIAGEN Cat # 74004 RNeasy Plus Micro Kit QIAGEN Cat # 74034 103 Genomics Chromium Single Cell Library Kit v2 103 Genomics Cat # 120234 RNAscope 2.5 LS Multiplex Reagent Kit Advanced Cell Diagnostics Cat # 322800 RNAscope LS 2.5 Probe- Mm-Gipr Advanced Cell Diagnostics Cat # 319128 RNAscope 2.5 LS Probe- Mm-Glp1r Advanced Cell Diagnostics Cat # 418858 RNAscope 3-plex LS Multiplex Control Positive Probe- Mm polr2A, ppib, ubc Advanced Cell Diagnostics Cat # 320888 RNAscope 3-plex LS Multiplex Negative Control Probe- dapB Advanced Cell Diagnostics Cat # 320878 RNAscope 2.5 LS Duplex Reagent Kit Advanced Cell Diagnostics Cat # 322440 RNAscope LS 2.5 Probe- Hs-GLP1R Advanced Cell Diagnostics Cat # 519828 RNAscope 2.5 LS Probe- Hs-GIPR Advanced Cell Diagnostics Cat # 471348 RNAscope 2.5 LS Positive Control Probe- Hs-PPIB Advanced Cell Diagnostics Cat # 313908 RNAscope 2.5 LS Duplex Negative Control Probe- DapB, DapB Advanced Cell Diagnostics Cat # 320758 (Continued on next page) Cell Metabolism 30, 987–996.e1–e6, November 5, 2019 e1

    Recombinant:

    Article Title: Glucose-Dependent Insulinotropic Polypeptide Receptor-Expressing Cells in the Hypothalamus Regulate Food Intake
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Goat Polyclonal anti-GLP-1 Santa Cruz Biotechnology Cat # sc-7782; RRID: AB_2107325 Guinea Pig Polycloal anti-insulin Abcam Cat # 7842; RRID: AB_306130 Goat Polyclonal anti-GFP Abcam Cat # 5450; RRID: AB_304897 Rabbit Polyclonal anti-DsRed Takara Bio Cat # 632496; RRID: AB_10013483 Alexa 488 secondary anti-goat Thermo Fisher Scientific Cat # A32814; RRID: AB_2762838 Alexa 555 secondary anti-rabbit Thermo Fisher Scientific Cat # A32794; RRID: AB_2762834 Alexa 633 secondary anti-guinea pig Thermo Fisher Scientific Cat # A-21105; RRID: AB_2535757 Biotinylated donkey anti-goat IgG Millipore Cat # AP180B; RRID: AB_11214009 Bacterial and Virus Strains AAV-hSyn-DIO-hM3D(Gq)-mCherry Addgene Cat # 44361-AAV8 Biological Samples Human hypothalamic brain blocks Cambridge Brain Bank https://www.cuh.nhs.uk/for-public/ cambridge-brain-bank Chemicals, Peptides, and Recombinant Proteins Papain Worthingon/ Lorne Labs Cat # LK003178 SuperScript III Reverse Transcriptase Thermo Fisher Scientific Cat # 18080093 SuperScript II Reverse Transcriptase Thermo Fisher Scientific Cat # 18064014 TaqMan Fast Universal PCR Master Mix Thermo Fisher Scientific Cat # 4364103 Glutamate Sigma Cat # G1251 CCK (octapeptide, sulfated) Tocris Cat # 1166 Hexarelin LKT Laboratories Cat # H1893 Leptin R&D Systems Cat # 498-OB-01M 5-HT Sigma Cat # H9523 Clozapine-N-Oxide Sigma Cat # C0832 Exendin-4 Tocris Cat # 1933 Critical Commercial Assays RNeasy Micro Kit QIAGEN Cat # 74004 RNeasy Plus Micro Kit QIAGEN Cat # 74034 103 Genomics Chromium Single Cell Library Kit v2 103 Genomics Cat # 120234 RNAscope 2.5 LS Multiplex Reagent Kit Advanced Cell Diagnostics Cat # 322800 RNAscope LS 2.5 Probe- Mm-Gipr Advanced Cell Diagnostics Cat # 319128 RNAscope 2.5 LS Probe- Mm-Glp1r Advanced Cell Diagnostics Cat # 418858 RNAscope 3-plex LS Multiplex Control Positive Probe- Mm polr2A, ppib, ubc Advanced Cell Diagnostics Cat # 320888 RNAscope 3-plex LS Multiplex Negative Control Probe- dapB Advanced Cell Diagnostics Cat # 320878 RNAscope 2.5 LS Duplex Reagent Kit Advanced Cell Diagnostics Cat # 322440 RNAscope LS 2.5 Probe- Hs-GLP1R Advanced Cell Diagnostics Cat # 519828 RNAscope 2.5 LS Probe- Hs-GIPR Advanced Cell Diagnostics Cat # 471348 RNAscope 2.5 LS Positive Control Probe- Hs-PPIB Advanced Cell Diagnostics Cat # 313908 RNAscope 2.5 LS Duplex Negative Control Probe- DapB, DapB Advanced Cell Diagnostics Cat # 320758 (Continued on next page) Cell Metabolism 30, 987–996.e1–e6, November 5, 2019 e1

    Reverse Transcription:

    Article Title: Glucose-Dependent Insulinotropic Polypeptide Receptor-Expressing Cells in the Hypothalamus Regulate Food Intake
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Goat Polyclonal anti-GLP-1 Santa Cruz Biotechnology Cat # sc-7782; RRID: AB_2107325 Guinea Pig Polycloal anti-insulin Abcam Cat # 7842; RRID: AB_306130 Goat Polyclonal anti-GFP Abcam Cat # 5450; RRID: AB_304897 Rabbit Polyclonal anti-DsRed Takara Bio Cat # 632496; RRID: AB_10013483 Alexa 488 secondary anti-goat Thermo Fisher Scientific Cat # A32814; RRID: AB_2762838 Alexa 555 secondary anti-rabbit Thermo Fisher Scientific Cat # A32794; RRID: AB_2762834 Alexa 633 secondary anti-guinea pig Thermo Fisher Scientific Cat # A-21105; RRID: AB_2535757 Biotinylated donkey anti-goat IgG Millipore Cat # AP180B; RRID: AB_11214009 Bacterial and Virus Strains AAV-hSyn-DIO-hM3D(Gq)-mCherry Addgene Cat # 44361-AAV8 Biological Samples Human hypothalamic brain blocks Cambridge Brain Bank https://www.cuh.nhs.uk/for-public/ cambridge-brain-bank Chemicals, Peptides, and Recombinant Proteins Papain Worthingon/ Lorne Labs Cat # LK003178 SuperScript III Reverse Transcriptase Thermo Fisher Scientific Cat # 18080093 SuperScript II Reverse Transcriptase Thermo Fisher Scientific Cat # 18064014 TaqMan Fast Universal PCR Master Mix Thermo Fisher Scientific Cat # 4364103 Glutamate Sigma Cat # G1251 CCK (octapeptide, sulfated) Tocris Cat # 1166 Hexarelin LKT Laboratories Cat # H1893 Leptin R&D Systems Cat # 498-OB-01M 5-HT Sigma Cat # H9523 Clozapine-N-Oxide Sigma Cat # C0832 Exendin-4 Tocris Cat # 1933 Critical Commercial Assays RNeasy Micro Kit QIAGEN Cat # 74004 RNeasy Plus Micro Kit QIAGEN Cat # 74034 103 Genomics Chromium Single Cell Library Kit v2 103 Genomics Cat # 120234 RNAscope 2.5 LS Multiplex Reagent Kit Advanced Cell Diagnostics Cat # 322800 RNAscope LS 2.5 Probe- Mm-Gipr Advanced Cell Diagnostics Cat # 319128 RNAscope 2.5 LS Probe- Mm-Glp1r Advanced Cell Diagnostics Cat # 418858 RNAscope 3-plex LS Multiplex Control Positive Probe- Mm polr2A, ppib, ubc Advanced Cell Diagnostics Cat # 320888 RNAscope 3-plex LS Multiplex Negative Control Probe- dapB Advanced Cell Diagnostics Cat # 320878 RNAscope 2.5 LS Duplex Reagent Kit Advanced Cell Diagnostics Cat # 322440 RNAscope LS 2.5 Probe- Hs-GLP1R Advanced Cell Diagnostics Cat # 519828 RNAscope 2.5 LS Probe- Hs-GIPR Advanced Cell Diagnostics Cat # 471348 RNAscope 2.5 LS Positive Control Probe- Hs-PPIB Advanced Cell Diagnostics Cat # 313908 RNAscope 2.5 LS Duplex Negative Control Probe- DapB, DapB Advanced Cell Diagnostics Cat # 320758 (Continued on next page) Cell Metabolism 30, 987–996.e1–e6, November 5, 2019 e1

    Polymerase Chain Reaction:

    Article Title: Glucose-Dependent Insulinotropic Polypeptide Receptor-Expressing Cells in the Hypothalamus Regulate Food Intake
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Goat Polyclonal anti-GLP-1 Santa Cruz Biotechnology Cat # sc-7782; RRID: AB_2107325 Guinea Pig Polycloal anti-insulin Abcam Cat # 7842; RRID: AB_306130 Goat Polyclonal anti-GFP Abcam Cat # 5450; RRID: AB_304897 Rabbit Polyclonal anti-DsRed Takara Bio Cat # 632496; RRID: AB_10013483 Alexa 488 secondary anti-goat Thermo Fisher Scientific Cat # A32814; RRID: AB_2762838 Alexa 555 secondary anti-rabbit Thermo Fisher Scientific Cat # A32794; RRID: AB_2762834 Alexa 633 secondary anti-guinea pig Thermo Fisher Scientific Cat # A-21105; RRID: AB_2535757 Biotinylated donkey anti-goat IgG Millipore Cat # AP180B; RRID: AB_11214009 Bacterial and Virus Strains AAV-hSyn-DIO-hM3D(Gq)-mCherry Addgene Cat # 44361-AAV8 Biological Samples Human hypothalamic brain blocks Cambridge Brain Bank https://www.cuh.nhs.uk/for-public/ cambridge-brain-bank Chemicals, Peptides, and Recombinant Proteins Papain Worthingon/ Lorne Labs Cat # LK003178 SuperScript III Reverse Transcriptase Thermo Fisher Scientific Cat # 18080093 SuperScript II Reverse Transcriptase Thermo Fisher Scientific Cat # 18064014 TaqMan Fast Universal PCR Master Mix Thermo Fisher Scientific Cat # 4364103 Glutamate Sigma Cat # G1251 CCK (octapeptide, sulfated) Tocris Cat # 1166 Hexarelin LKT Laboratories Cat # H1893 Leptin R&D Systems Cat # 498-OB-01M 5-HT Sigma Cat # H9523 Clozapine-N-Oxide Sigma Cat # C0832 Exendin-4 Tocris Cat # 1933 Critical Commercial Assays RNeasy Micro Kit QIAGEN Cat # 74004 RNeasy Plus Micro Kit QIAGEN Cat # 74034 103 Genomics Chromium Single Cell Library Kit v2 103 Genomics Cat # 120234 RNAscope 2.5 LS Multiplex Reagent Kit Advanced Cell Diagnostics Cat # 322800 RNAscope LS 2.5 Probe- Mm-Gipr Advanced Cell Diagnostics Cat # 319128 RNAscope 2.5 LS Probe- Mm-Glp1r Advanced Cell Diagnostics Cat # 418858 RNAscope 3-plex LS Multiplex Control Positive Probe- Mm polr2A, ppib, ubc Advanced Cell Diagnostics Cat # 320888 RNAscope 3-plex LS Multiplex Negative Control Probe- dapB Advanced Cell Diagnostics Cat # 320878 RNAscope 2.5 LS Duplex Reagent Kit Advanced Cell Diagnostics Cat # 322440 RNAscope LS 2.5 Probe- Hs-GLP1R Advanced Cell Diagnostics Cat # 519828 RNAscope 2.5 LS Probe- Hs-GIPR Advanced Cell Diagnostics Cat # 471348 RNAscope 2.5 LS Positive Control Probe- Hs-PPIB Advanced Cell Diagnostics Cat # 313908 RNAscope 2.5 LS Duplex Negative Control Probe- DapB, DapB Advanced Cell Diagnostics Cat # 320758 (Continued on next page) Cell Metabolism 30, 987–996.e1–e6, November 5, 2019 e1

    RNAscope:

    Article Title: Glucose-Dependent Insulinotropic Polypeptide Receptor-Expressing Cells in the Hypothalamus Regulate Food Intake
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Goat Polyclonal anti-GLP-1 Santa Cruz Biotechnology Cat # sc-7782; RRID: AB_2107325 Guinea Pig Polycloal anti-insulin Abcam Cat # 7842; RRID: AB_306130 Goat Polyclonal anti-GFP Abcam Cat # 5450; RRID: AB_304897 Rabbit Polyclonal anti-DsRed Takara Bio Cat # 632496; RRID: AB_10013483 Alexa 488 secondary anti-goat Thermo Fisher Scientific Cat # A32814; RRID: AB_2762838 Alexa 555 secondary anti-rabbit Thermo Fisher Scientific Cat # A32794; RRID: AB_2762834 Alexa 633 secondary anti-guinea pig Thermo Fisher Scientific Cat # A-21105; RRID: AB_2535757 Biotinylated donkey anti-goat IgG Millipore Cat # AP180B; RRID: AB_11214009 Bacterial and Virus Strains AAV-hSyn-DIO-hM3D(Gq)-mCherry Addgene Cat # 44361-AAV8 Biological Samples Human hypothalamic brain blocks Cambridge Brain Bank https://www.cuh.nhs.uk/for-public/ cambridge-brain-bank Chemicals, Peptides, and Recombinant Proteins Papain Worthingon/ Lorne Labs Cat # LK003178 SuperScript III Reverse Transcriptase Thermo Fisher Scientific Cat # 18080093 SuperScript II Reverse Transcriptase Thermo Fisher Scientific Cat # 18064014 TaqMan Fast Universal PCR Master Mix Thermo Fisher Scientific Cat # 4364103 Glutamate Sigma Cat # G1251 CCK (octapeptide, sulfated) Tocris Cat # 1166 Hexarelin LKT Laboratories Cat # H1893 Leptin R&D Systems Cat # 498-OB-01M 5-HT Sigma Cat # H9523 Clozapine-N-Oxide Sigma Cat # C0832 Exendin-4 Tocris Cat # 1933 Critical Commercial Assays RNeasy Micro Kit QIAGEN Cat # 74004 RNeasy Plus Micro Kit QIAGEN Cat # 74034 103 Genomics Chromium Single Cell Library Kit v2 103 Genomics Cat # 120234 RNAscope 2.5 LS Multiplex Reagent Kit Advanced Cell Diagnostics Cat # 322800 RNAscope LS 2.5 Probe- Mm-Gipr Advanced Cell Diagnostics Cat # 319128 RNAscope 2.5 LS Probe- Mm-Glp1r Advanced Cell Diagnostics Cat # 418858 RNAscope 3-plex LS Multiplex Control Positive Probe- Mm polr2A, ppib, ubc Advanced Cell Diagnostics Cat # 320888 RNAscope 3-plex LS Multiplex Negative Control Probe- dapB Advanced Cell Diagnostics Cat # 320878 RNAscope 2.5 LS Duplex Reagent Kit Advanced Cell Diagnostics Cat # 322440 RNAscope LS 2.5 Probe- Hs-GLP1R Advanced Cell Diagnostics Cat # 519828 RNAscope 2.5 LS Probe- Hs-GIPR Advanced Cell Diagnostics Cat # 471348 RNAscope 2.5 LS Positive Control Probe- Hs-PPIB Advanced Cell Diagnostics Cat # 313908 RNAscope 2.5 LS Duplex Negative Control Probe- DapB, DapB Advanced Cell Diagnostics Cat # 320758 (Continued on next page) Cell Metabolism 30, 987–996.e1–e6, November 5, 2019 e1

    Multiplex Assay:

    Article Title: Glucose-Dependent Insulinotropic Polypeptide Receptor-Expressing Cells in the Hypothalamus Regulate Food Intake
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Goat Polyclonal anti-GLP-1 Santa Cruz Biotechnology Cat # sc-7782; RRID: AB_2107325 Guinea Pig Polycloal anti-insulin Abcam Cat # 7842; RRID: AB_306130 Goat Polyclonal anti-GFP Abcam Cat # 5450; RRID: AB_304897 Rabbit Polyclonal anti-DsRed Takara Bio Cat # 632496; RRID: AB_10013483 Alexa 488 secondary anti-goat Thermo Fisher Scientific Cat # A32814; RRID: AB_2762838 Alexa 555 secondary anti-rabbit Thermo Fisher Scientific Cat # A32794; RRID: AB_2762834 Alexa 633 secondary anti-guinea pig Thermo Fisher Scientific Cat # A-21105; RRID: AB_2535757 Biotinylated donkey anti-goat IgG Millipore Cat # AP180B; RRID: AB_11214009 Bacterial and Virus Strains AAV-hSyn-DIO-hM3D(Gq)-mCherry Addgene Cat # 44361-AAV8 Biological Samples Human hypothalamic brain blocks Cambridge Brain Bank https://www.cuh.nhs.uk/for-public/ cambridge-brain-bank Chemicals, Peptides, and Recombinant Proteins Papain Worthingon/ Lorne Labs Cat # LK003178 SuperScript III Reverse Transcriptase Thermo Fisher Scientific Cat # 18080093 SuperScript II Reverse Transcriptase Thermo Fisher Scientific Cat # 18064014 TaqMan Fast Universal PCR Master Mix Thermo Fisher Scientific Cat # 4364103 Glutamate Sigma Cat # G1251 CCK (octapeptide, sulfated) Tocris Cat # 1166 Hexarelin LKT Laboratories Cat # H1893 Leptin R&D Systems Cat # 498-OB-01M 5-HT Sigma Cat # H9523 Clozapine-N-Oxide Sigma Cat # C0832 Exendin-4 Tocris Cat # 1933 Critical Commercial Assays RNeasy Micro Kit QIAGEN Cat # 74004 RNeasy Plus Micro Kit QIAGEN Cat # 74034 103 Genomics Chromium Single Cell Library Kit v2 103 Genomics Cat # 120234 RNAscope 2.5 LS Multiplex Reagent Kit Advanced Cell Diagnostics Cat # 322800 RNAscope LS 2.5 Probe- Mm-Gipr Advanced Cell Diagnostics Cat # 319128 RNAscope 2.5 LS Probe- Mm-Glp1r Advanced Cell Diagnostics Cat # 418858 RNAscope 3-plex LS Multiplex Control Positive Probe- Mm polr2A, ppib, ubc Advanced Cell Diagnostics Cat # 320888 RNAscope 3-plex LS Multiplex Negative Control Probe- dapB Advanced Cell Diagnostics Cat # 320878 RNAscope 2.5 LS Duplex Reagent Kit Advanced Cell Diagnostics Cat # 322440 RNAscope LS 2.5 Probe- Hs-GLP1R Advanced Cell Diagnostics Cat # 519828 RNAscope 2.5 LS Probe- Hs-GIPR Advanced Cell Diagnostics Cat # 471348 RNAscope 2.5 LS Positive Control Probe- Hs-PPIB Advanced Cell Diagnostics Cat # 313908 RNAscope 2.5 LS Duplex Negative Control Probe- DapB, DapB Advanced Cell Diagnostics Cat # 320758 (Continued on next page) Cell Metabolism 30, 987–996.e1–e6, November 5, 2019 e1

    Control:

    Article Title: Glucose-Dependent Insulinotropic Polypeptide Receptor-Expressing Cells in the Hypothalamus Regulate Food Intake
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Goat Polyclonal anti-GLP-1 Santa Cruz Biotechnology Cat # sc-7782; RRID: AB_2107325 Guinea Pig Polycloal anti-insulin Abcam Cat # 7842; RRID: AB_306130 Goat Polyclonal anti-GFP Abcam Cat # 5450; RRID: AB_304897 Rabbit Polyclonal anti-DsRed Takara Bio Cat # 632496; RRID: AB_10013483 Alexa 488 secondary anti-goat Thermo Fisher Scientific Cat # A32814; RRID: AB_2762838 Alexa 555 secondary anti-rabbit Thermo Fisher Scientific Cat # A32794; RRID: AB_2762834 Alexa 633 secondary anti-guinea pig Thermo Fisher Scientific Cat # A-21105; RRID: AB_2535757 Biotinylated donkey anti-goat IgG Millipore Cat # AP180B; RRID: AB_11214009 Bacterial and Virus Strains AAV-hSyn-DIO-hM3D(Gq)-mCherry Addgene Cat # 44361-AAV8 Biological Samples Human hypothalamic brain blocks Cambridge Brain Bank https://www.cuh.nhs.uk/for-public/ cambridge-brain-bank Chemicals, Peptides, and Recombinant Proteins Papain Worthingon/ Lorne Labs Cat # LK003178 SuperScript III Reverse Transcriptase Thermo Fisher Scientific Cat # 18080093 SuperScript II Reverse Transcriptase Thermo Fisher Scientific Cat # 18064014 TaqMan Fast Universal PCR Master Mix Thermo Fisher Scientific Cat # 4364103 Glutamate Sigma Cat # G1251 CCK (octapeptide, sulfated) Tocris Cat # 1166 Hexarelin LKT Laboratories Cat # H1893 Leptin R&D Systems Cat # 498-OB-01M 5-HT Sigma Cat # H9523 Clozapine-N-Oxide Sigma Cat # C0832 Exendin-4 Tocris Cat # 1933 Critical Commercial Assays RNeasy Micro Kit QIAGEN Cat # 74004 RNeasy Plus Micro Kit QIAGEN Cat # 74034 103 Genomics Chromium Single Cell Library Kit v2 103 Genomics Cat # 120234 RNAscope 2.5 LS Multiplex Reagent Kit Advanced Cell Diagnostics Cat # 322800 RNAscope LS 2.5 Probe- Mm-Gipr Advanced Cell Diagnostics Cat # 319128 RNAscope 2.5 LS Probe- Mm-Glp1r Advanced Cell Diagnostics Cat # 418858 RNAscope 3-plex LS Multiplex Control Positive Probe- Mm polr2A, ppib, ubc Advanced Cell Diagnostics Cat # 320888 RNAscope 3-plex LS Multiplex Negative Control Probe- dapB Advanced Cell Diagnostics Cat # 320878 RNAscope 2.5 LS Duplex Reagent Kit Advanced Cell Diagnostics Cat # 322440 RNAscope LS 2.5 Probe- Hs-GLP1R Advanced Cell Diagnostics Cat # 519828 RNAscope 2.5 LS Probe- Hs-GIPR Advanced Cell Diagnostics Cat # 471348 RNAscope 2.5 LS Positive Control Probe- Hs-PPIB Advanced Cell Diagnostics Cat # 313908 RNAscope 2.5 LS Duplex Negative Control Probe- DapB, DapB Advanced Cell Diagnostics Cat # 320758 (Continued on next page) Cell Metabolism 30, 987–996.e1–e6, November 5, 2019 e1

    Negative Control:

    Article Title: Glucose-Dependent Insulinotropic Polypeptide Receptor-Expressing Cells in the Hypothalamus Regulate Food Intake
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Goat Polyclonal anti-GLP-1 Santa Cruz Biotechnology Cat # sc-7782; RRID: AB_2107325 Guinea Pig Polycloal anti-insulin Abcam Cat # 7842; RRID: AB_306130 Goat Polyclonal anti-GFP Abcam Cat # 5450; RRID: AB_304897 Rabbit Polyclonal anti-DsRed Takara Bio Cat # 632496; RRID: AB_10013483 Alexa 488 secondary anti-goat Thermo Fisher Scientific Cat # A32814; RRID: AB_2762838 Alexa 555 secondary anti-rabbit Thermo Fisher Scientific Cat # A32794; RRID: AB_2762834 Alexa 633 secondary anti-guinea pig Thermo Fisher Scientific Cat # A-21105; RRID: AB_2535757 Biotinylated donkey anti-goat IgG Millipore Cat # AP180B; RRID: AB_11214009 Bacterial and Virus Strains AAV-hSyn-DIO-hM3D(Gq)-mCherry Addgene Cat # 44361-AAV8 Biological Samples Human hypothalamic brain blocks Cambridge Brain Bank https://www.cuh.nhs.uk/for-public/ cambridge-brain-bank Chemicals, Peptides, and Recombinant Proteins Papain Worthingon/ Lorne Labs Cat # LK003178 SuperScript III Reverse Transcriptase Thermo Fisher Scientific Cat # 18080093 SuperScript II Reverse Transcriptase Thermo Fisher Scientific Cat # 18064014 TaqMan Fast Universal PCR Master Mix Thermo Fisher Scientific Cat # 4364103 Glutamate Sigma Cat # G1251 CCK (octapeptide, sulfated) Tocris Cat # 1166 Hexarelin LKT Laboratories Cat # H1893 Leptin R&D Systems Cat # 498-OB-01M 5-HT Sigma Cat # H9523 Clozapine-N-Oxide Sigma Cat # C0832 Exendin-4 Tocris Cat # 1933 Critical Commercial Assays RNeasy Micro Kit QIAGEN Cat # 74004 RNeasy Plus Micro Kit QIAGEN Cat # 74034 103 Genomics Chromium Single Cell Library Kit v2 103 Genomics Cat # 120234 RNAscope 2.5 LS Multiplex Reagent Kit Advanced Cell Diagnostics Cat # 322800 RNAscope LS 2.5 Probe- Mm-Gipr Advanced Cell Diagnostics Cat # 319128 RNAscope 2.5 LS Probe- Mm-Glp1r Advanced Cell Diagnostics Cat # 418858 RNAscope 3-plex LS Multiplex Control Positive Probe- Mm polr2A, ppib, ubc Advanced Cell Diagnostics Cat # 320888 RNAscope 3-plex LS Multiplex Negative Control Probe- dapB Advanced Cell Diagnostics Cat # 320878 RNAscope 2.5 LS Duplex Reagent Kit Advanced Cell Diagnostics Cat # 322440 RNAscope LS 2.5 Probe- Hs-GLP1R Advanced Cell Diagnostics Cat # 519828 RNAscope 2.5 LS Probe- Hs-GIPR Advanced Cell Diagnostics Cat # 471348 RNAscope 2.5 LS Positive Control Probe- Hs-PPIB Advanced Cell Diagnostics Cat # 313908 RNAscope 2.5 LS Duplex Negative Control Probe- DapB, DapB Advanced Cell Diagnostics Cat # 320758 (Continued on next page) Cell Metabolism 30, 987–996.e1–e6, November 5, 2019 e1

    Positive Control:

    Article Title: Glucose-Dependent Insulinotropic Polypeptide Receptor-Expressing Cells in the Hypothalamus Regulate Food Intake
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Goat Polyclonal anti-GLP-1 Santa Cruz Biotechnology Cat # sc-7782; RRID: AB_2107325 Guinea Pig Polycloal anti-insulin Abcam Cat # 7842; RRID: AB_306130 Goat Polyclonal anti-GFP Abcam Cat # 5450; RRID: AB_304897 Rabbit Polyclonal anti-DsRed Takara Bio Cat # 632496; RRID: AB_10013483 Alexa 488 secondary anti-goat Thermo Fisher Scientific Cat # A32814; RRID: AB_2762838 Alexa 555 secondary anti-rabbit Thermo Fisher Scientific Cat # A32794; RRID: AB_2762834 Alexa 633 secondary anti-guinea pig Thermo Fisher Scientific Cat # A-21105; RRID: AB_2535757 Biotinylated donkey anti-goat IgG Millipore Cat # AP180B; RRID: AB_11214009 Bacterial and Virus Strains AAV-hSyn-DIO-hM3D(Gq)-mCherry Addgene Cat # 44361-AAV8 Biological Samples Human hypothalamic brain blocks Cambridge Brain Bank https://www.cuh.nhs.uk/for-public/ cambridge-brain-bank Chemicals, Peptides, and Recombinant Proteins Papain Worthingon/ Lorne Labs Cat # LK003178 SuperScript III Reverse Transcriptase Thermo Fisher Scientific Cat # 18080093 SuperScript II Reverse Transcriptase Thermo Fisher Scientific Cat # 18064014 TaqMan Fast Universal PCR Master Mix Thermo Fisher Scientific Cat # 4364103 Glutamate Sigma Cat # G1251 CCK (octapeptide, sulfated) Tocris Cat # 1166 Hexarelin LKT Laboratories Cat # H1893 Leptin R&D Systems Cat # 498-OB-01M 5-HT Sigma Cat # H9523 Clozapine-N-Oxide Sigma Cat # C0832 Exendin-4 Tocris Cat # 1933 Critical Commercial Assays RNeasy Micro Kit QIAGEN Cat # 74004 RNeasy Plus Micro Kit QIAGEN Cat # 74034 103 Genomics Chromium Single Cell Library Kit v2 103 Genomics Cat # 120234 RNAscope 2.5 LS Multiplex Reagent Kit Advanced Cell Diagnostics Cat # 322800 RNAscope LS 2.5 Probe- Mm-Gipr Advanced Cell Diagnostics Cat # 319128 RNAscope 2.5 LS Probe- Mm-Glp1r Advanced Cell Diagnostics Cat # 418858 RNAscope 3-plex LS Multiplex Control Positive Probe- Mm polr2A, ppib, ubc Advanced Cell Diagnostics Cat # 320888 RNAscope 3-plex LS Multiplex Negative Control Probe- dapB Advanced Cell Diagnostics Cat # 320878 RNAscope 2.5 LS Duplex Reagent Kit Advanced Cell Diagnostics Cat # 322440 RNAscope LS 2.5 Probe- Hs-GLP1R Advanced Cell Diagnostics Cat # 519828 RNAscope 2.5 LS Probe- Hs-GIPR Advanced Cell Diagnostics Cat # 471348 RNAscope 2.5 LS Positive Control Probe- Hs-PPIB Advanced Cell Diagnostics Cat # 313908 RNAscope 2.5 LS Duplex Negative Control Probe- DapB, DapB Advanced Cell Diagnostics Cat # 320758 (Continued on next page) Cell Metabolism 30, 987–996.e1–e6, November 5, 2019 e1



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    Journal: Cell Metabolism

    Article Title: Glucose-Dependent Insulinotropic Polypeptide Receptor-Expressing Cells in the Hypothalamus Regulate Food Intake

    doi: 10.1016/j.cmet.2019.07.013

    Figure Lengend Snippet:

    Article Snippet: RNAscope® 3-plex LS Multiplex Negative Control Probe- dapB , Advanced Cell Diagnostics , Cat # 320878.

    Techniques: Virus, Recombinant, Reverse Transcription, RNAscope, Multiplex Assay, Control, Negative Control, Positive Control, Expressing, Isolation, Derivative Assay, Gene Expression, Software